Optimizing 3D spheroid monoculture and co-culture models of breast cancer for pharmaceutical investigation and screening

• Suárez

  English

  Author

• Josém

  English

  Author

• de Medina

  English

  Author

 Test methods utilized in preclinical cancer medication development must have 
reproducibility and physiological relevance. In this regard, the ability of 3D cell culture models, such as 
spheroids or organoids, to replicate natural biology has recently made them appealing. Additionally, 
complete automation of sample preparation and data processing is crucial to boosting screening efficiency 
and throughput.OBJECTIVE: This work addressed spheroid cell culture methodological factors, such as 
media composition, extracellular matrix, addition of stromal cells, and quality of contrast-based readouts, 
that are particularly relevant for reproducibility and physiological significance in studies investigating 
metabolic effects of drug treatment. 

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