UPLC: A PREEMINENT PHARMACEUTICAL ANALYSIS TECHNIQUE

• Guo Tang

  English

  Author

• Xiao Feng

  English

  Author

 Today's pharmaceutical businesses are motivated to develop new and more 
effective methods for medication discovery, development, delivery, and monitoring. The 
creation of a quick chromatographic process is essential for analytical labs in this 
competition. Significant technological advancements have been made in the last ten years 
in the areas of particle chemistry performance, detector design, system optimization, data 
processing, and other chromatographic method controls. Ultra-high performance liquid 
chromatography (UPLC), which holds back the premise of HPLC technique, produced 
exceptional performance when all was combined. By utilizing particles smaller than 2 µm 
and operating at higher pressure, UPLC exhibits a significant improvement in speed, 
resolution, and sensitivity of analysis, and the mobile phase may be able to run at higher 
linear velocities than HPLC. In the field of liquid chromatographic investigations, this 
approach is regarded as a new focal point. The fundamental principles, equipment, and 
benefits of UPLC over HPLC are the main topics of this review. Additionally, this article 
highlights the numerous pharmaceutical uses of this method. 

• pdf