Development and Validation of Stability Indicating HPTLC Method for Estimation of Rizatriptan Benzoate in Bulk and Tablet Dosage Form

• P Ramanarasimham

  English

  Author

For the determination of rizatiptan benzoate in bulk and tablet formulation, a novel, straightforward,
accurate, precise, and selective stability-indicating high performance thin layer chromatographic
(HPTLC) approach has been created and validated. Using methanol: n-propane:triethylamine (3:5:2 v/v/v)
as the mobile phase and densitometric scanning at 280 nm, chromatographic separation was carried out
on an aluminum plate that had been precoated with Silica Gel 60 F254. Rizatiptan benzoate was shown to
have a compact spot in this system (Rf value of Rf = 0.38 ± 0.02). It was discovered that the calibration
curve was linear between 800-2800 ng/band. It was discovered that the limits of detection and quantitation
were 2.24 ng/band and 6.79 ng/band, respectively. During stability investigations, the suggested approach
may be used to estimate Rizatiptan Benzoate.

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