A Validated Rp-Hplc Method for Estimation of Ciprofloxacin and Tinidazole in Tablet Dosage Form

• N Krishna

  English

  Author

For the estimation of ciprofloxacin and tinidazole in combination tablet dose form, a straightforward,
specific, accurate, and exact Reverse Phase High Performance Liquid Chromatography (RP-HPLC)
approach was created and validated. Using an Inertsil C18 (250X4.6mm) column and mobile phase
phosphate buffers (pH 6.8): acetonitrile (82:18) v/v, the separation was performed at a flow rate of 1.0
ml/min. At 316 nm, detection was done. Ciprofloxacin and tinidazole were shown to have retention times
of 5.6 and 9.82 minutes, respectively. The linearity, accuracy, and precision of the procedure were
confirmed. The linearity of ciprofloxacin and tinidazole was found to be between 27.5 and 82.5μg/ml for
ciprofloxacin and between 33μg/ml and 66μg/ml for tinidazole, with a correlation coefficient of 0.9999
for both medications. Ciprofloxacin and tinidazole were reported to have mean recovery rates of 99.7 and
100.4, respectively. Tinidazole and ciprofloxacin in combination tablet dose form may be routinely
analyzed using the described approach

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