Impact on Living Things Penetration of the Tetra-Branched Anti-TNF- Peptide Dependent on the Coating Ratio Transtympanic Injection of Gamma-Maghemite Nanoparticles Stabilized by Conjugated Cerium3/4+ and Visualized by Magnetic Resonance Imaging (MRI) into

• Kettunen UI

  English

  Author

• Lsellouche

  English

  Author

• Pyykköhj I

  English

  Author

Goal: After a transtympanic injection, determine the distribution of TBATP in the inner ear and assess their biological effectiveness.
Approach: The TBATP was produced by means of the conventional Fmoc solid phase synthesis. It was in human umbilical vein
endothelial cells (HUVECs) that a model of TNF-α-induced apoptosis was developed. The CAN-γ-Fe2O3 NPs were covalently
linked to the peptides. The middle ear cavity of rats was injected with a suspension of nanoparticles. A 7.0 T MRI scanner, in
conjunction with Prussian blue staining, was used to determine the distribution of CAN-γ-Fe2O3 NPs in the inner ear.
Findings: The inhibitory impact on HUVECs caused by TNF-α was almost entirely mitigated by TBATP, in contrast to the linear one,
which only mitigated half of the effect. At 3 hours and 2 days after administration to the middle ear, CAN-γ-Fe2O3 NPs conjugated to
TBATP at a peptide weight ratio of 10% (but not 50%) effectively reached the inner ear, with the effect being most noticeable at 2
days.

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