MiR-590-5P Inhibits HepG2 Cell Growth by Inhibiting the Wnt Pathway and Reducing S100A10 Expression

• C Dichev

  English

  Author

• D Dicheva

  English

  Author

One of the most prevalent and deadly tumors in the world, particularly in underdeveloped
nations, is hepatocellular carcinoma. In the current investigation, we discovered that six hepatocellular
carcinoma cell lines had up-regulated S100A10 expression and down-regulated miR-590-5P expression.
According to the reporter gene test, luciferase generated by a vector containing the 3' untranslated region
of S100A10 mRNA was much less active when miR-590-5P was overexpressed. S100A10 expression
was reduced by lentiviral infection-induced ectopic miR-590-5P overexpression. HepG2 cells that were
infected with Lv-miR-590-5P experienced cell growth inhibition and cell cycle G1 arrest. Furthermore,
the expression of miR-590-5P enhanced the phosphorylation of β-catenin and the production of Caspase 3,
while suppressing the expression of Wnt5a, cMyc, and cyclin D1. These effects may be part of the
mechanism by which miR-590-5P inhibits cell growth. When combined, our findings imply that
hepatocellular carcinoma is associated with down-regulation of miR-590-5P and that restoring miR-590-
5P might inhibit the proliferation of cancer cells, indicating that miR-590-5P may be a viable target
molecule for hepatocellular carcinoma treatment.

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