Iron Oxide Nanoparticle Biosynthesis using Lactobacillus Fermentum Bacterial Cytoplasmic Extract

• Fsdereshte Ghandehari

  English

  Author

• Malahat Rezaeejh

  English

  Author

The manufacturing of nanoparticles has occupied much of the scientific and technological community's attention during the last decade. Nanoparticle
synthesis may be accomplished in a number of ways, however these methods are inefficient and expensive. Consequently, biologic methods for
producing nanoparticles are required. Nanoparticles are manufactured using a variety of biological structures, including plants, algae, and microbes
including bacteria, string molds, and yeasts. The aim of this research is to employ green chemistry as a basis for the manufacture of iron oxide
nanoparticles using cytoplasmic extracts of the probiotic microbe Lactobacillus Fermentum. We incubated the bacterial cytoplasmic extract of
Lactobacillus Fermentum for three weeks at 37 °C with 5% carbon dioxide after preparing it using the freez-thow technique. We then added an equal
volume ratio of iron sulfate solution (III) at a concentration of 10-3 M. The use of X-ray diffraction (XRD) and transmission electron microscopy
(TEM) allowed for the investigation of nanoparticle production. As soon as iron sulfate nanoparticles begin to form, the solution's color will change to
black. The XRD examination demonstrated that the cytoplasmic extract of Lactobacillus Fermentum could generate nano crystals of iron oxide.
According to the results of the transmission electron microscopy (TEM) analysis, the typical size of the nanoparticles was 10-15 nm, and they had a
spherical form. When it comes to producing iron oxide nanoparticles, there is an efficient biological process that is both cost-effective and ecologically
benign. This method involves using Lactobacillus fermentum cytoplasmic extract.

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